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c8 d30  (ATCC)


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    ATCC c8 d30
    C8 D30, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crl+2534/us12612438-423-13-14?v=ATCC
    Average 94 stars, based on 50 article reviews
    c8 d30 - by Bioz Stars, 2026-08
    94/100 stars

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    Figure 1. Expression of PROKR1 and PROKR2 proteins in b.End.3, <t>C8-D30,</t> and N2a cell lines. (A) Representative Western blot analysis of PROKR1 expression in b.End.3 (endothelial cells), C8-D30 <t>(astrocytes),</t> and N2a (neurons) cells. (B) Quantification of PROKR1 expression levels. (C) Represen- tative Western blot analysis of PROKR2 expression in the three cell lines, b.End.3, C8-D30, and N2a. (D) Quantification of PROKR2 expression levels. Quantification was performed with Image-J (3.53). Data are presented as mean ± SEM, from three independent experiments.
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    Figure 1. Expression of PROKR1 and PROKR2 proteins in b.End.3, C8-D30, and N2a cell lines. (A) Representative Western blot analysis of PROKR1 expression in b.End.3 (endothelial cells), C8-D30 (astrocytes), and N2a (neurons) cells. (B) Quantification of PROKR1 expression levels. (C) Represen- tative Western blot analysis of PROKR2 expression in the three cell lines, b.End.3, C8-D30, and N2a. (D) Quantification of PROKR2 expression levels. Quantification was performed with Image-J (3.53). Data are presented as mean ± SEM, from three independent experiments.

    Journal: International journal of molecular sciences

    Article Title: Effects of Prokineticins on Cerebral Cell Function and Blood-Brain Barrier Permeability.

    doi: 10.3390/ijms242015428

    Figure Lengend Snippet: Figure 1. Expression of PROKR1 and PROKR2 proteins in b.End.3, C8-D30, and N2a cell lines. (A) Representative Western blot analysis of PROKR1 expression in b.End.3 (endothelial cells), C8-D30 (astrocytes), and N2a (neurons) cells. (B) Quantification of PROKR1 expression levels. (C) Represen- tative Western blot analysis of PROKR2 expression in the three cell lines, b.End.3, C8-D30, and N2a. (D) Quantification of PROKR2 expression levels. Quantification was performed with Image-J (3.53). Data are presented as mean ± SEM, from three independent experiments.

    Article Snippet: We used murine brain microvascular endothelial cells (bEnd.3 [BEND3] (ATCC CRL-2299, LGC, Molsheim, France)), murine astrocytes (C8-D30 [Astrocyte type III clone] (ATCC CRL2534, LGC, Molsheim, France)), DMEM high glucose (#41965-039 GIBCO, ThermoFisher Scientific, Waltham, MA, USA), and N2a murine Neuroblastoma (ATCC CCL-131) DMEM Glutamax (#31966-021 GIBCO, ThermoFisher Scientific).

    Techniques: Expressing, Western Blot

    Figure 2. Effect of PROK1 and PROK2 on the proliferation of b.End3, C8-D30, and N2a cell lines. Effect of PROK1 on the proliferation of (A) endothelial cells (b.End.3), (B) astrocytes (C8-D30), and (C) neurons (N2a). Effect of PROK2 on the proliferation of (D) endothelial cells, (E) astrocytes, and (F) neurons. Data are presented as mean ± SEM, from three independent experiments; unpaired two-tailed t test was used for statistical analysis and statistical significance is indicated as * p ≤0.05, ** p ≤0.01, *** p ≤0.001, compared to the condition without prokineticin.

    Journal: International journal of molecular sciences

    Article Title: Effects of Prokineticins on Cerebral Cell Function and Blood-Brain Barrier Permeability.

    doi: 10.3390/ijms242015428

    Figure Lengend Snippet: Figure 2. Effect of PROK1 and PROK2 on the proliferation of b.End3, C8-D30, and N2a cell lines. Effect of PROK1 on the proliferation of (A) endothelial cells (b.End.3), (B) astrocytes (C8-D30), and (C) neurons (N2a). Effect of PROK2 on the proliferation of (D) endothelial cells, (E) astrocytes, and (F) neurons. Data are presented as mean ± SEM, from three independent experiments; unpaired two-tailed t test was used for statistical analysis and statistical significance is indicated as * p ≤0.05, ** p ≤0.01, *** p ≤0.001, compared to the condition without prokineticin.

    Article Snippet: We used murine brain microvascular endothelial cells (bEnd.3 [BEND3] (ATCC CRL-2299, LGC, Molsheim, France)), murine astrocytes (C8-D30 [Astrocyte type III clone] (ATCC CRL2534, LGC, Molsheim, France)), DMEM high glucose (#41965-039 GIBCO, ThermoFisher Scientific, Waltham, MA, USA), and N2a murine Neuroblastoma (ATCC CCL-131) DMEM Glutamax (#31966-021 GIBCO, ThermoFisher Scientific).

    Techniques: Two Tailed Test

    Figure 3. Effect of PROK1 and PROK2 on the migration of b.End.3, C8-D30, and N2a cell lines. Effect of PROK1 on the migration of (A) endothelial cells (b.End.3), (B) astrocytes (C8-D30), and (C) neurons (N2a). Effect of PROK2 on the migration of (D) endothelial cells, (E) astrocytes, and (F) neurons. Data are presented as mean ± SEM, from three independent experiments; unpaired two-tailed t test was used for statistical analysis and statistical significance is indicated as * p ≤0.05, ** p ≤0.01, *** p ≤0.001 compared to the condition without prokineticin.

    Journal: International journal of molecular sciences

    Article Title: Effects of Prokineticins on Cerebral Cell Function and Blood-Brain Barrier Permeability.

    doi: 10.3390/ijms242015428

    Figure Lengend Snippet: Figure 3. Effect of PROK1 and PROK2 on the migration of b.End.3, C8-D30, and N2a cell lines. Effect of PROK1 on the migration of (A) endothelial cells (b.End.3), (B) astrocytes (C8-D30), and (C) neurons (N2a). Effect of PROK2 on the migration of (D) endothelial cells, (E) astrocytes, and (F) neurons. Data are presented as mean ± SEM, from three independent experiments; unpaired two-tailed t test was used for statistical analysis and statistical significance is indicated as * p ≤0.05, ** p ≤0.01, *** p ≤0.001 compared to the condition without prokineticin.

    Article Snippet: We used murine brain microvascular endothelial cells (bEnd.3 [BEND3] (ATCC CRL-2299, LGC, Molsheim, France)), murine astrocytes (C8-D30 [Astrocyte type III clone] (ATCC CRL2534, LGC, Molsheim, France)), DMEM high glucose (#41965-039 GIBCO, ThermoFisher Scientific, Waltham, MA, USA), and N2a murine Neuroblastoma (ATCC CCL-131) DMEM Glutamax (#31966-021 GIBCO, ThermoFisher Scientific).

    Techniques: Migration, Two Tailed Test

    Figure 5. Assessment of transendothelial electrical resistance (TEER) and FD-40 passage in monolayer and co-culture models. (A) TEER measurements of endothelial cell monolayer and endothelial with astrocyte co-culture models were recorded over a 7-day culture period, with values presented in ohm.cm2. (B) The reduction of spontaneous passage of FD-40 across the endothelial monolayer and co-culture models compared to the spontaneous migration of FD-40 across the insert without cells. Data are presented as mean ± SEM, and representative of three independent experiments. Statistical analysis: two-way ANOVA corrected by Sidak test for multiple comparisons was used for TEER measurements and unpaired one-tailed t-test was used for FD-40 passage measurements. Statistical significance is indicated as * p < 0.05 and # p = 0.1.

    Journal: International journal of molecular sciences

    Article Title: Effects of Prokineticins on Cerebral Cell Function and Blood-Brain Barrier Permeability.

    doi: 10.3390/ijms242015428

    Figure Lengend Snippet: Figure 5. Assessment of transendothelial electrical resistance (TEER) and FD-40 passage in monolayer and co-culture models. (A) TEER measurements of endothelial cell monolayer and endothelial with astrocyte co-culture models were recorded over a 7-day culture period, with values presented in ohm.cm2. (B) The reduction of spontaneous passage of FD-40 across the endothelial monolayer and co-culture models compared to the spontaneous migration of FD-40 across the insert without cells. Data are presented as mean ± SEM, and representative of three independent experiments. Statistical analysis: two-way ANOVA corrected by Sidak test for multiple comparisons was used for TEER measurements and unpaired one-tailed t-test was used for FD-40 passage measurements. Statistical significance is indicated as * p < 0.05 and # p = 0.1.

    Article Snippet: We used murine brain microvascular endothelial cells (bEnd.3 [BEND3] (ATCC CRL-2299, LGC, Molsheim, France)), murine astrocytes (C8-D30 [Astrocyte type III clone] (ATCC CRL2534, LGC, Molsheim, France)), DMEM high glucose (#41965-039 GIBCO, ThermoFisher Scientific, Waltham, MA, USA), and N2a murine Neuroblastoma (ATCC CCL-131) DMEM Glutamax (#31966-021 GIBCO, ThermoFisher Scientific).

    Techniques: Co-Culture Assay, Migration, One-tailed Test